Information that Watson and Crick used to propose the double helix model: 1) R. Watson and Crick (1953) Watson and Crick (1953): “It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material”. 1950: “It is, however, noteworthy -whether this is more than accidental, cannot yet be said-that in all deoxypentose nucleic acids examined thus far the molar ratios of total purines to total pyrimidines, and also of adenine to thymine and of guanine to cytosine, were not far from 1”. Rosalind Franklin (1950 or 1951) Chargaff. Links 3’-O of preceding nucleotide to 5’ of next nucleotide ->5’-3’ polarity.DNA Sequencing 6- Principles of Nucleic Acids Denaturation.Nucleic Acids Structures 1-Discovery of DNA structure 2- A, B and Z conformations of dsDNA/dsRNA 3- DNA tertiary Structures Nucleosome, G-quadruplex 4- Secondary and tertiary Structure of RNA 5- Principles of DNA Recognition by sequence-specific DNA binding proteins
0 kommentar(er)
